Dear Dr. Byrne
We are submitting the article: "DlgS97 is essential for short term memory in Drosophila", authored by Carlos Oliva and Jimena Sierralta, so that you consider its publication Learning and Memory.
We believe that this study is potentially interesting for Learning and Memory readers, because it shows, for the first time an in vivo role of Dlg in the memory storage; since the complete mutation of dlg gene is lethal, precluding any intent of performing this kind of study. We used a mutant of a specific splicing variant that knocks out just one of the two products of the dlg gene. This mutant is viable and has not defects in the basic brain architecture.
The manuscript has been written and approved by both authors.
This study has not been submitted for publication elsewhere, and to our knowledge there is no conflict of interests.
Sincerely
Carlos Oliva PhD Student
Physiology and Biophysics department
Faculty of Medicine
University of Chile
Ph: 56-2-9786033
coliva@med.uchile.cl
jueves, 17 de junio de 2010
jueves, 10 de junio de 2010
Commentary about creativity
In my point of view Mr. Roberson is right in several ways; however we have to be carefully and do not make the inverse mistake. I mean, put all the children in some kind of school only for arts. I think creativity is not only in arts, but also in mathematics and science. Maybe a good way to improve the current model is to implement an educational system, where in the first years the teachers find what talents the children have and then use specific curses to potentiate them.
In respect of the article creativity and mental health, it is very interesting. But I think that should be more factors involve that just expression of dopamine receptors. Indeed seems like that the activity of the Thalamus is the key factor. Any way the article support the general notion that people very creative is also a little crazy.
In respect of the article creativity and mental health, it is very interesting. But I think that should be more factors involve that just expression of dopamine receptors. Indeed seems like that the activity of the Thalamus is the key factor. Any way the article support the general notion that people very creative is also a little crazy.
Cover letter 1
Dear Dr. Byrne
We are submitting our article: "DlgS97 is essential for short term memory in Drosophila", by the authors Carlos Oliva and Jimena Sierralta, so that you consider its publication in your prestigious journal Learning and Memory. The manuscript has been written and approved by all of the authors. Our study is not under consideration at any other journal for publication and we have not conflict of interest.
We think that our study is potentially interesting for Learning and Memory readers, because it shows for the first time an in vivo role of Dlg in the memory storage. Since the complete mutation of dlg gene is lethal, precluding any intent of perform this kind of study, we used a mutant of a specific splicing variant that knock out just one of the two products of the dlg gene. This mutant is viable and has not defects in the basic brain architecture.
Sincerely
Carlos Oliva PhD Student
Physiology and Biophysics department
Faculty of Medicine
University of Chile
We are submitting our article: "DlgS97 is essential for short term memory in Drosophila", by the authors Carlos Oliva and Jimena Sierralta, so that you consider its publication in your prestigious journal Learning and Memory. The manuscript has been written and approved by all of the authors. Our study is not under consideration at any other journal for publication and we have not conflict of interest.
We think that our study is potentially interesting for Learning and Memory readers, because it shows for the first time an in vivo role of Dlg in the memory storage. Since the complete mutation of dlg gene is lethal, precluding any intent of perform this kind of study, we used a mutant of a specific splicing variant that knock out just one of the two products of the dlg gene. This mutant is viable and has not defects in the basic brain architecture.
Sincerely
Carlos Oliva PhD Student
Physiology and Biophysics department
Faculty of Medicine
University of Chile
jueves, 13 de mayo de 2010
Final Abstract
DLGS97 is essential for short term memory in Drosophila.
The synaptic membrane associated guanylate kinase (MAGUK) scaffold protein family is thought to play key role in synapse assembly, synaptic plasticity and memory. Evidence supporting these roles in vivo is still missing. Previous research has showed that a mutant of Drosophila DLGS97 (homolog of mammalian MAGUK protein SAP97) presents alterations in complex behaviors without obvious developmental defects. The aim of this study was to assess whether DLGS97 is involved in the learning process. Here we show that DLGS97 mutants had no alterations in general brain morphology and normal distribution and quantity of neurons and glial cells. We also showed that morphology of mushroom bodies, the structures involved in the memory storage in the fly, were normal and the subcellular distribution of the protein is pre and postsynaptic in these cells. Finally we performed a learning test in the DLGS97 mutants and we found a deficiency in a short term memory task. Our results indicate that DLGS97 is not necessary for Mushroom Body morphogenesis but it is essential for short term memory.
Keywords: DLG, Learning, Short term memory, Drosophila.
The synaptic membrane associated guanylate kinase (MAGUK) scaffold protein family is thought to play key role in synapse assembly, synaptic plasticity and memory. Evidence supporting these roles in vivo is still missing. Previous research has showed that a mutant of Drosophila DLGS97 (homolog of mammalian MAGUK protein SAP97) presents alterations in complex behaviors without obvious developmental defects. The aim of this study was to assess whether DLGS97 is involved in the learning process. Here we show that DLGS97 mutants had no alterations in general brain morphology and normal distribution and quantity of neurons and glial cells. We also showed that morphology of mushroom bodies, the structures involved in the memory storage in the fly, were normal and the subcellular distribution of the protein is pre and postsynaptic in these cells. Finally we performed a learning test in the DLGS97 mutants and we found a deficiency in a short term memory task. Our results indicate that DLGS97 is not necessary for Mushroom Body morphogenesis but it is essential for short term memory.
Keywords: DLG, Learning, Short term memory, Drosophila.
jueves, 6 de mayo de 2010
Task3
DLGS97 is essential for short term memory in Drosophila
The synaptic associated guanylate kinase (MAGUK) scaffold protein family is thought to play key roles in synapse assembly, synaptic plasticity and memory. Evidence supporting these roles in vivo is still missing. Previous research showed that a mutant of Drosophila DLGS97 (homolog of mammalian MAGUK protein SAP97) presents alterations in complex behaviors like phototaxis and circadian rhythm without obvious developmental defects. The aim of this study was to assess the importance DLGS97 protein in learning. Here we showed that DLGS97 mutants had no alterations in general morphology of the brain and normal distribution and quantity of neurons and glial cells. We also showed that morphology of Mushroom Bodies, the structures involved in the memory storage in the fly, were normal and the subcellular distribution of the protein is pre and postsynaptic in these cells. Finally we performed a learning test in the DLGS97 mutants and we found a deficiency in the 5 minutes learning task. Our results indicate that DLGS97 is not necessary for Mushroom Bodies morphogenesis but it is essential for short term memory.
The synaptic associated guanylate kinase (MAGUK) scaffold protein family is thought to play key roles in synapse assembly, synaptic plasticity and memory. Evidence supporting these roles in vivo is still missing. Previous research showed that a mutant of Drosophila DLGS97 (homolog of mammalian MAGUK protein SAP97) presents alterations in complex behaviors like phototaxis and circadian rhythm without obvious developmental defects. The aim of this study was to assess the importance DLGS97 protein in learning. Here we showed that DLGS97 mutants had no alterations in general morphology of the brain and normal distribution and quantity of neurons and glial cells. We also showed that morphology of Mushroom Bodies, the structures involved in the memory storage in the fly, were normal and the subcellular distribution of the protein is pre and postsynaptic in these cells. Finally we performed a learning test in the DLGS97 mutants and we found a deficiency in the 5 minutes learning task. Our results indicate that DLGS97 is not necessary for Mushroom Bodies morphogenesis but it is essential for short term memory.
jueves, 29 de abril de 2010
Task2
DLGS97 is essential for learning in Drosophila
The synaptic associated guanylate kinase (MAGUK) scaffold protein family is thought to play key roles in synapse assembly, synaptic plasticity and memory, but evidence supporting these roles in vivo is still missing. In a previous work of our lab we had shown that a Drosophila homolog of mammalian MAGUK proteins SAP97, DLGS97 mutant presents alterations in complex behaviors like phototaxis and circadian rhythm without obvious developmental defects. Our objective in this work was test whether DLGS97 is important for learning. Here we showed that DLGS97 mutants had no alterations in general morphology of the brain and normal distribution and quantity of neurons and glial cells. We also showed that morphology of Mushroom Bodies, the structures involved in the memory storage in the fly, were normal and the subcellular distribution of the protein is pre and postsynaptic in these cells. Finally we performed a learning test in the DLGS97 mutants and we found a deficiency in the 5 minutes learning task. Our results indicate that DLGS97 is not necessary for Mushroom Bodies morphogenesis but it is essential for learning.
The synaptic associated guanylate kinase (MAGUK) scaffold protein family is thought to play key roles in synapse assembly, synaptic plasticity and memory, but evidence supporting these roles in vivo is still missing. In a previous work of our lab we had shown that a Drosophila homolog of mammalian MAGUK proteins SAP97, DLGS97 mutant presents alterations in complex behaviors like phototaxis and circadian rhythm without obvious developmental defects. Our objective in this work was test whether DLGS97 is important for learning. Here we showed that DLGS97 mutants had no alterations in general morphology of the brain and normal distribution and quantity of neurons and glial cells. We also showed that morphology of Mushroom Bodies, the structures involved in the memory storage in the fly, were normal and the subcellular distribution of the protein is pre and postsynaptic in these cells. Finally we performed a learning test in the DLGS97 mutants and we found a deficiency in the 5 minutes learning task. Our results indicate that DLGS97 is not necessary for Mushroom Bodies morphogenesis but it is essential for learning.
miércoles, 21 de abril de 2010
Work1
I am Carlos Oliva. I studied Biochemistry in the Pontificia Universidad Católica de Chile. Now I am
in the Biomedical Science PhD program in the Faculty of Medicine of Universidad de Chile. My subject
of interest is neurobiology, specifically the mechanisms involved in the axon growth and guidance
during the development of neuron system. In my lab we use the fly Drosophila melanogaster, because
it is powerful genetic model and we use it for discover new genes involved in the neuron
development. When I will finish my thesis I would like to do a postdoc in a laboratory abroad for
get more experience in the use of Drosophila in the study human diseases.
in the Biomedical Science PhD program in the Faculty of Medicine of Universidad de Chile. My subject
of interest is neurobiology, specifically the mechanisms involved in the axon growth and guidance
during the development of neuron system. In my lab we use the fly Drosophila melanogaster, because
it is powerful genetic model and we use it for discover new genes involved in the neuron
development. When I will finish my thesis I would like to do a postdoc in a laboratory abroad for
get more experience in the use of Drosophila in the study human diseases.
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